AccuPower RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low copy RNA or mRNA and subsequent RT-PCR .
Features and Benefits
| Convenient lyophilized RT: | Easy to use, simply add your purified RNA |
| High yield of cDNA: | For genes up to 6 kb |
| Stable for 2 years at -20°C: | Long life |
| RNase, DNase and Proteinase-free: | Ensures the integrity of your samples |
Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 6 kb
Application
cDNA synthesis followed by PCR
Figure 1. Specific amplification of 5' - UTR region of HCV with AccuPower RT-PCR PreMix. Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.
Lane 1: 123 DNA Ladder
Lane 2: Negative control
Lane 3, 4: HCV positive serum
Lane 5: HCV negative serum
